The cyanobacterium known as Anabaena variabilis is an organism composed of a multicellular, it has a unique ability to differentiate into specialized heterocysts that are specifically geared towards nitrogen fixation. These dormant cells allow the organism to survive under an extensive range of environmentally induced stresses. The objective of the present study is to investigate the impact of abiotic stresses, namely nitrogen (N), phosphorus (P), and sulphur (S), on the axenic culture of Anabaena variabilis and its production of pigments such as chl a and carotenoids, as well as metabolites like proteins, carbohydrates, phenols, and flavonoids. The growth rate was evaluated using optical density (OD)750 nm, and the nutrient supplementation of nitrogen (N), phosphorus (P), and sulphur (S) at 2.5 g/L, 0.06 g/L, and 0,095g/L, respectively, resulted in the highest algal dry weight and maximum production of pigments and biochemical productivity. This signifies that the growth of A. variabilis when subjected to varying nutritional compositions results in the alteration of the cultivation constituents' composition.