The highly toxic systemic neonicotinoid insecticide Acetamiprid may seriously damage aquatic ecosystems and human health when it reaches into water bodies. Therefore, there is an urgent need to create environmentally friendly, inexpensive, and efficient adsorbents to remove Acetamiprid from effluents. The current study presents research on converting Moringa oleifera seed waste (MOS) obtained from oil industry wastes into beneficial nano sorbent. In order to maximize the removal of Acetamiprid from contaminated wastewater, adsorption study examined the effects of solution pH, agitation time, Acetamiprid concentration, and temperature. The adsorption equilibrium was modeled with Langmuir isotherm. The adsorption kinetics was modeled with power function and Elovich equation. Depending on Langmuir models, maximum adsorption capacity for nano sorbent reached to 70.22 mg·g-1, which is 5.8 times higher than qmax of bulk MOS. According to the thermodynamic analysis, Acetamiprid adsorption occurs spontaneously and exothermically. The three main mechanisms of adsorption on nMOS were found to be hydrogen bonding, electrostatic interlinkage, and π–π interactions. The sorption capacity in the first four cycles of the adsorption-desorption investigated a slight decrease from 94% to 88%, indicating the effective reuse of nMOS in Acetamiprid removal from effluents. Overall, the experimental findings revealed that nMOS is a low-cost, environmentally friendly, and promising nanosorbent that may be used to treat Acetamiprid-contaminated effluents.