Antibiotic resistance is one of the most severe global public health threats; it can kill 700,000 people and could rise to ten million by 2050 so the study includes detection the antibiotic resistant, the infection of the urinary tract is one of the predominant infections among people that result from pathogenic bacteria. More than 80% of UTI instances are brought on by a bacterium, and the most common culprit is E. coli, which is a typical component of the intestinal flora. The most common gram-negative bacterium in the study is E. coli about 25 %, K. pneumonia about 12 %, Enterobacter aerogenes about 10 % and others while the most common gram-positive bacteria that cause UTI is S. aureus 12 %, S. epidermidis about 8 % and Enterococcus faecalis about 4 %. the bacterial Isolates were discovered by morphological, biochemical and VITEK-2 compact system. The study includes detection the antibiotic-resistant by VITEK-2 compact system and the antibiotic-resistant gene for detect bacteria that contain the most antibiotic-resistant gene and eventually lead to modification in the future. The gene used in the study is (TEM found at 41%, GES at 41 %, and OXA at 50 %) the three resistant genes found in K. pneumonia, E. coli and P. aeruginosa but the other bacteria also contain one of the tested genes such as TEM found in K. oxytoca and P.mirabilis, GES in Enterobacter aerogenes and Citrobacter freundii, while the OXA gene in A. baumanii, C .freundii and K. oxytoca.