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351673

ANTIOXIDANT CAPACITY OF CYSTEAMINE ON BUFFALO GRANULOSA CELL PERFORMANCE UNDER OXIDATIVE STRESS CONDITIONS

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Physiology

Abstract

The impact of antioxidant supplementation of cysteamine during in vitro culture of buffalo granulosa cells (GCs) was evaluated in relation to their viability and enzymatic activities under hydrogen peroxide (H2O2)-induced oxidative stress. Mural GCs were harvested, cultured for 24 h of confluence at 37.5ºC and then exposed to 500 µM H2O2 for 30 min followed by cysteamine supplementation (0, 100, 200, 300 and 400 µM) for further 24 h of culture and their counterpart-control groups were supplemented with cysteamine without H2O2 pre-treatment. Granulosa cell viability was assessed using neutral red assay, while the culture conditioned media were subjected to enzymatic analyses. Our results revealed that GCs viability rate was decreased by H2O2 pre-treatment; being 63.0±14.0, 61.8±14.1, and 61.8±10.3 % for 100, 300, and 400 µM of cysteamine, respectively, except increasing viability for 200µM cysteamine (80.0±10.0 %) as compared to control (65.2±4.0). However, the viability was insignificantly enhanced by cysteamine levels under normal physiological conditions. For enzymatic analyses, comparable GSH-Px activity was monitored for all groups of the study. Catalase activity was consistent in 100 and 200 µM of cysteamine under H2O2 or normal physiological condition. While a significant reduction was observed in 300 µM of cysteamine under H2O2 pre-treatment, there were no significant differences between all groups of the study for malondialdehyde (MDA) levels. In conclusion, H2O2 negatively affected GCs viability; however, 200 µM of cysteamine ameliorated cells viability under oxidative stressful condition. The antioxidant enzymes GSH-Px/CAT acted in synergistic dynamics against oxidative stress.

DOI

10.21608/ejap.2024.250268.1065

Keywords

granulosa cells, Oxidative Stress, Cysteamine, Hydrogen Peroxide, GSH-Px

Authors

First Name

Marwa

Last Name

Faheem

MiddleName

-

Affiliation

Animal Production Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt

Email

marwasf@agr.cu.edu.eg

City

Giza

Orcid

0000-0002-3999-5098

First Name

Dalia

Last Name

Ahmed

MiddleName

-

Affiliation

Animal Production Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt

Email

dodyaa26@yahoo.com

City

Giza

Orcid

-

First Name

Sherif

Last Name

Dessouki

MiddleName

-

Affiliation

Animal Production Department, Faculty of Agriculture, Cairo University, 12613, Giza, Egypt

Email

sherifd2002@agr.cu.edu.eg

City

Giza

Orcid

-

Volume

61

Article Issue

1

Related Issue

46979

Issue Date

2024-01-01

Receive Date

2023-11-27

Publish Date

2024-01-01

Page Start

31

Page End

37

Print ISSN

0302-4520

Online ISSN

2735-3028

Article File

EJAP_Volume 61_Issue 1_Pages 31-37.pdf

PDF . 755.8kB

Link

https://ejap.journals.ekb.eg/article_351673.html

Detail API

https://ejap.journals.ekb.eg/service?article_code=351673

Order

4

Type

Original Article

Type Code

1,298

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Production

Publication Link

https://ejap.journals.ekb.eg/

MainTitle

ANTIOXIDANT CAPACITY OF CYSTEAMINE ON BUFFALO GRANULOSA CELL PERFORMANCE UNDER OXIDATIVE STRESS CONDITIONS

Details

Type

Article

Created At

25 Dec 2024