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315238

DETECTING THE MOLECULAR ASSOCIATIONS OF PROLACTIN GENE GENOTYPES WITH MILK TRAITS IN FRIESIAN AND BALADI COWS USING RFLP TECHNIQUE

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Animal genetic and breeding

Abstract

Four years of experimental work were conducted applying PCR-RFLP to detect the SNP polymorphic associations of the prolactin candidate gene (PRL) with milk traits in cattle. A total of 494 lactations for 180 milking cows of Friesian in Elkarda herd (FK) and Sakha herd (FS) and Baladi cows in Elserw herd (BS) were used. The PCR products were digested using NaeI and SmaI restricted enzymes to investigate the molecular associations of the PRL gene genotypes with milk traits.       The highest expected numbers of alleles (NE) in CC, CD and DD genotypes of the PRL gene were recorded in the DD genotype in both Friesian and Baladi herds, while the lowest expected numbers were recorded in CC genotype in all herds. The highest NE in GG, GT and TT genotype was in the TT genotype and the lowest NE was in the GG genotype in all herds. PRL gene was not in Hardy-Weinberg Equilibrium in all herds. The allelic frequency for the D allele was much higher than the frequency of the C allele, while the frequency of the T allele was higher than the frequency of the G allele.       The observed and expected values of heterozygosity for the PRL gene were mostly moderate in the three herds studied. The values of polymorphic information content (PIC) were mostly moderate in all herds. The values of fixation index (reduction in heterozygosity due to inbreeding, FIS) were high in the FK herd (0.82), FS herd (0.62) and BS herd (0.94).                 The molecular associations between PRL gene genotypes and milk traits indicate that the CD genotype of the PRL gene has strong molecular associations with yields of milk, fat and protein relative to CC and DD genotypes. GG genotype was significantly associated with all milk traits relative to GT and TT genotypes

DOI

10.21608/ejap.2023.219284.1060

Keywords

Friesian and Baladi cows, PRL gene, PCR-RFLP, polymorphic associations, milk yield and its compositions

Authors

First Name

Maher

Last Name

Khalil

MiddleName

-

Affiliation

Department of Animal Production, Faculty of Agriculture at Moshtohor, Benha University, 13736 Tukh, Kalyoubia Governorate, Egypt

Email

maher.khalil@fagr.bu.edu.eg

City

New Cairo

Orcid

0000-0002-6929-3011

First Name

M

Last Name

Iraqi

MiddleName

M

Affiliation

Department of Animal Production, Faculty of Agriculture at Moshtohor, Benha University, 13736 Tukh, Kalyoubia Governorate, Egypt

Email

-

City

-

Orcid

-

First Name

M

Last Name

Mohamed

MiddleName

H

Affiliation

Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

S

Last Name

Zahed

MiddleName

M

Affiliation

Animal Production Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt

Email

-

City

-

Orcid

-

Volume

60

Article Issue

2

Related Issue

42806

Issue Date

2023-05-01

Receive Date

2023-06-25

Publish Date

2023-05-01

Page Start

77

Page End

86

Print ISSN

0302-4520

Online ISSN

2735-3028

Article File

EJAP_Volume 60_Issue 2_Pages 77-86.pdf

PDF . 648.9kB

Link

https://ejap.journals.ekb.eg/article_315238.html

Detail API

https://ejap.journals.ekb.eg/service?article_code=315238

Order

4

Type

Original Article

Type Code

1,298

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Production

Publication Link

https://ejap.journals.ekb.eg/

MainTitle

DETECTING THE MOLECULAR ASSOCIATIONS OF PROLACTIN GENE GENOTYPES WITH MILK TRAITS IN FRIESIAN AND BALADI COWS USING RFLP TECHNIQUE

Details

Type

Article

Created At

25 Dec 2024