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311759

SUPEROXIDE DISMUTASE MIMICS )TEMPOL) IMPROVES IN VITRO OOCYTES MATURATION IN EGYPTIAN BUFFALOES

Article

Last updated: 25 Dec 2024

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Subjects

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Tags

Physiology

Abstract

The purpose of this study was to examine how Tempol, an exogenous antioxidant, affected the in vitro maturation of Egyptian buffalo oocytes at the molecular level. Cumulus-oocyte complexes (COCs) were recovered from the ovaries of slaughtered animals. Cumulus-oocyte complexes were matured in a maturation medium supplemented with different concentrations of Tempol (0, 0.5, 1, and 2 μM)2-24 h and then nuclear maturation and cumulus cells expansion rates were evaluated. Profiled the expression of two candidate genes regulating metabolic activity and antioxidant status were analyzed in the matured oocytes using Real-Time PCR. Tempol increased the rate of buffalo COC expansion. The nuclear maturation rate was significantly improved in the 0.5 μM group compared to the 1 and 2 μM groups and the control group. A significant decline in the transcriptional abundance of CPT2 was observed with the increase in the Tempol concentration in the maturation medium. While, the expression level of the antioxidant-related gene showed a lower level of expression of different genes used in comparison with all experimental groups. In conclusion, the 0.5 μM concentration of Tempol may improve oocyte maturation and molecular competence

DOI

10.21608/ejap.2023.205754.1059

Keywords

Tempol, in vitro maturation, Buffalo, oocytes

Authors

First Name

Osama

Last Name

Sakr

MiddleName

Galal

Affiliation

Animal Production Department, Faculty of Agriculture, Cairo Univerisity

Email

osamasakr@gmail.com

City

Giza

Orcid

0000-0001-9586-8686

Volume

60

Article Issue

2

Related Issue

42806

Issue Date

2023-05-01

Receive Date

2023-04-13

Publish Date

2023-05-01

Page Start

43

Page End

48

Print ISSN

0302-4520

Online ISSN

2735-3028

Article File

EJAP_Volume 60_Issue 2_Pages 43-48.pdf

PDF . 442.9kB

Link

https://ejap.journals.ekb.eg/article_311759.html

Detail API

https://ejap.journals.ekb.eg/service?article_code=311759

Order

1

Type

Original Article

Type Code

1,298

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Production

Publication Link

https://ejap.journals.ekb.eg/

MainTitle

SUPEROXIDE DISMUTASE MIMICS )TEMPOL) IMPROVES IN VITRO OOCYTES MATURATION IN EGYPTIAN BUFFALOES

Details

Type

Article

Created At

25 Dec 2024