The present study examines the effect of financial inclusion on banks' profitability in both developing countries (low-income) and developed countries (high-income). The study took place over the period form 2010-2022. We find that banks' profitability in both developed and developing countries is affected by financial inclusion. The study sample consists of 10 countries as five low-income countries and five high-income countries. The study uncovers the existence of a positive effect of number of ATMs on banks' return on equity in both developed and developing countries. And the bank branches have a negative effect on banks' return on assets and banks' return on equity in both developed and developing countries, this may be due to the increasing cost of establishing these branches. There may be more influential variables, but this does not prevent that financial inclusion still has an effect. There may be more influential variables, but this does not prevent that financial inclusion still has an effect.