Kidney failure is a disease that affects the entire body and often leads to death. Samples were collected from Al-Sadr Teaching Hospital, Kidney Unit, from patients with renal failure for the period from 1/1/2024 to 1/6/2024. The number of samples was 28. Also, 28 people were collected from a control group. The samples were examined in the laboratories of the College of Science, University of Kufa. Consent was obtained from the patients according to the agreement between the University of Kufa and Al-Sadr Teaching Hospital. The average age of the patients was (45.24±1.3) while the age of the control group was (38.31±2.4). The result shows a significant increase in creatinine, urea, and total CO2 in people with renal failure compared to the control group (mean ± standard error). The results also showed a significant decrease in eGFR and vitamin D3. The study concluded that kidney dysfunction first affects the functions of other cells and causes other parameters to lose their functions, ultimately leading to death.