With the high incidence and mortality rates associated with complications of viral hepatitis B, including cancer and cirrhosis, infection with this disease remains a health problem throughout the world. This study aims to assess the levels of knowledge regarding Hepatitis B among medical technical students at the Mosul Medical Technical Institute in Mosul City, Iraq. Utilizing a convenience sampling method, a total of 300 students aged 18-41 years participated in the study. A structured questionnaire, validated by a panel of experts, was employed to evaluate participants' knowledge about HBV transmission, symptoms, and preventive measures. The results indicated that 89% of students possessed acceptable knowledge about Hepatitis B, while 7% demonstrated good knowledge, and 4% had poor knowledge. Key findings revealed that the highest awareness was related to the necessity of conducting HBV tests before surgical procedures (79%) and the importance of vaccination (68%). However, knowledge regarding the avoidance of contaminated water was notably low (5%). The study underscores the critical need for enhanced educational interventions to improve awareness and understanding of Hepatitis B among future healthcare professionals, thereby contributing to better prevention and control of this infectious disease.