Celiac disease is an autoimmune disorder that occurs when people with a genetic predisposition react to gluten, a protein found in wheat, barley, and rye. This reaction primarily affects the small intestine, causing a variety of symptoms. While the disease is most commonly associated with digestive issues, it can also cause problems in other parts of the body, leading to both intestinal and non-intestinal symptoms. This condition can affect both children and adults, triggering an immune response that results in damage to the intestine. The study aims to increase the role of (pro-inflammatory cytokines IL-6) and (anti-inflammatory cytokines IL-10 and IL-21) in the serum of CD patients. A total of 103 individuals of both sexes with an age range of 5-≥ 64 years were collected from (AL-Sadder Medical City in Najaf province). All patients were examined serologically by TTG antibody (IgG & IgA) and antigliadin (IgG & IgA) with a special ELISA kit for determination of CD patients. Interlukin-6, interleukin-10, and interleukin-21 were examined serologically in patients (103) and healthy persons as a control group. The findings of the current study indicate that there was no significant elevation of IL-6(30.98±24.58) and IL-10 (60.32 ± 17.97) in celiac disease patients comparable with control (27.4±15.66) and (51.6±18.64), respectively. IL-21 revealed a significant (P ≤ 0.001) elevation (100.26±27.77) in CD patients compared to control (58.7±20.02).