Background: In 50% of cases, the pathogenesis of unexplained recurrent Spontaneous abortion (URSA) is still unclear. ` We assess the αvbeta3 integrin gene expression in fertile and RSA-afflicted women. Methods: Endometrial tissues (biopsies) (30 samples) divided into 15 RSA cases and controls (15) healthy through menstrual cycle in the mid-luteal period. The period of data collection was March 2022–December 2023. The αvß3 gene expression was examined and in compression to fertile women using (qRT-PCR). Results: The relative gene expression of αvß3 integrin was significantly decreased (p-value < 0.05) in RSA women in compression to control subjects. The reduction of αvß3 integrin might have an essential role in the pathogenesis of RSA. Conclusion: Comparing women with unexplained RSA to controls, the expression of integrin was considerably lower. Our results highlight the necessity of additional molecular examination of the endometrial tissue in afflicted women.