Background: The current study aims to diagnose fungi isolated from wounds using traditional and molecular methods, study virulence factors, and test biofilm formation. Chronic wound infection results from several factors such as repeated infections, chronic diseases, or disorders of the immune system. Materials and methods: This research included 250 patients in Kirkuk City. Direct laboratory examination of the samples was performed using 10% KOH, and laboratory examination after culturing the samples on Sabouraud Dextrose Agar medium. Results: Three types of yeast were diagnosed Candida albicans, Candida, tropicalis, and Candida krusei. The most frequent of them was C. albicans, with 27 isolates (29.67%) C. tropicalis 22 isolates 24.18%, and C. krusei  7 isolates 7.69%. The diagnosis of the isolated yeasts was confirmed based on PCR technology.
Conclusions: The study showed that diabetic foot wound infection was the most common and that Candida spp. is the primary cause of these infections.