To prepare a culture medium for the growth of Leishmania parasite consisting of two phases in the laboratory, bee pollen was used, and the bee pollen and misshapen blood were placed with added dextrose and agar in the medium. To prepare the liquid medium, Bee pollen filtrate was used instead of Locke's solution as the liquid phase, and for comparison, Locke's solution was used at the same time. In all media, the highest rate of parasite numbers reached on the eighth day of growth, (10⁶ x 19.55  cells/ml) with a media containing (3.7 g) of Bee pollen in the solid phase, either The second reading: 10⁶ x 20.95 cells/ml with a media containing (3.7 g) of filtrate grains in the liquid phase, and the third reading: 10⁶ x 19.37 cells/ml in a medium containing (3.7 g) of Bee pollen in the solid phase and (3.7 g) from the filtrate of the grain represents the liquid phase. In the NNN medium and the liquid phase was only oral rehydration solution the fourth reading: was 10⁶ x 20.37 cells/ml. The parasite continued to exist in the medium in lower numbers but with good vitality for twenty days, where the number of parasites was recorded at (0.62, 1.62, 0.82, 1.12) x 10⁶ cells/ml Sequentially.
The parasite's vitality increases from the second day and reaches its highest levels on the eighth day.