Context: Acrylic resin denture base material is the most commonly used dental material for removable prostheses, although its high affinity to form biofilms on its surface results in serious health problems. Aims: Isolation and identification of aerobic bacteria and Candida from biofilms formed on denture base surfaces. Quantifying the biofilm-forming ability and studying the anti-adherence effect of Polyethylene glycol (PEG) and chlorohexidine diacetate salt (CHX) added to heat-cured denture base material. Methods and Material: Microbial isolates were collected from denture base surfaces, and further identified by morphological, and biochemical tests, VITEK-2 system, and DNA sequencing. The biofilm-forming potential was examined by Microtiter Plate Assay. The anti-adherence effect of PEG and CHX added to denture base materials was evaluated. Results: The investigated microorganisms had different biofilm-forming potential on denture base surfaces ranging through weak, moderate and strong. The PEG and CHX had antibacterial and anti-fungal potential, and the addition of PEG and CHX to denture base resin significantly decreased the adhesion of selected microorganisms compared with control groups. The statistical results were considered significant at p ≤ 0.05.
Conclusions: This study revealed that modifying denture base resin by the addition of PEG and CHX significantly enhances the anti-adherence property. The combining of PEG and CHX resulted in the enhanced decline of microorganism adherence.