Asymptomatic Bacteriuria (ASB) is a pathological state distinguished by substantial urinary infection in the absence of any observable symptoms. ASB is a result of the presence of typical gut microorganisms that migrate via the urethra and potentially reach the bladder and kidneys. The diagnosis relies on urine culture, genetic analysis using The Polymerase Chain Reaction (PCR), and bacteriophage administration as a therapeutic intervention. Methods: The examination was conducted as a prospective study at Mosul Laboratories. The study lasted for one month, A total of 100 participants, aged between 15 and 33, were recruited, including representatives from both sexes. All individuals involved in the study were clinically verified to display no indications or manifestations of Urinary Tract Infection (UTI). Results: The study involved 100 volunteers and cultured urine samples. Bacterial isolates were identified using the Vitek-2 System and PCR methods. Bacteria prevalence was 84%, higher among 15-20 year olds. GPB species were more prevalent in females, with 61% of isolated cases being Gram-Positive Bacteria (GPB) and 23% being Gram-Negative Bacteria (GNB). which was utilized to do sensitivity testing on different antibiotics utilizing Antimicrobial Susceptibility Testing (AST) cards. Identified species included Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Conclusion: Non-pregnant women and healthy males frequently experience ASB. Regular urine culture sensitivity trying should be finished for people of all ages corporations and sexes to mitigate the ability dangers and outcomes connected with this condition.