Cholecystectomy, the most popular surgical procedure worldwide, is carried out laparoscopically in more than 90% of cases and is considered as "gold standard" for the surgical treatment of gallstones. Aim of the study: Our research set out to determine whether women's levels of estrogen and progesterone hormones were related to cholecystectomy. Methods and Materials: 40 women, 20 of whom had undergone cholecystectomy and 20 as a control group and were aged 30-45, participated examined Al-Sadr Teaching Hospital and a few laboratories in Misan City between November 2023 to February 2024. The participants gave up 5 ml of their whole blood via a medical syringe. The blood sample was put in a gel tube and placed at room temperature for 20 min to allow for clotting and centrifuged for 10 minutes at 3000 rpm to collect serum to test the hormones Estro and Progs. Results. When compared to the patients' Progesterone decreased significantly (p ≤0.050) in the patients' group (0.41 ± 0.40 ng/mL) compared with the control group (1.75 ± 0.95 ng /mL). When compared to patients(5.54±4.49), the Estro findings in the control group(45.68±23.88) were considerably higher(p ≤0.05).Conclusion: In the present study we can conclude the levels of progesterone and estrogen decreased in cholecystectomy women because the women that choices in our study were nonpregnant and did not use contraceptive therapy, and the risk of cholecystectomy increased after 6 months of gallbladder removal, and it increased in pregnant women more than nonpregnant women.