Risk factors for cardiovascular disease (CVD) include hyperglycemia, high blood pressure, a high body mass index (BMI), and hypercholesterolemia. The two sexes have an increased risk of CHD with age, but the rise has been found greater in women. The research aimed to assess the effects of sex and age on parameters of amylin, preptin, SOD, MDA, fasting blood glucose (FBG), and CAT in CVD patients without and with type 2 diabetes (T2D) in comparison to healthy individuals.  There were 150 participants in the study: 50 normal volunteers, or control subjects who were thought to be healthy, and 100 patients with cardiovascular disease, of which 50 had type 2 diabetes and the remaining 50 did not. Participants in the study varied in age from 40 to 70 years old and had type 2 diabetes and CVD but were not obese. The study's findings demonstrated a substantial relationship between sex and the concentrations of CAT, preptin, amylin, MDA, and SOD. Age affects the parameters that are evaluated in patients with cardiovascular disease, including those with and those without type 2 diabetes. The primary cardiovascular risk factors have accounted for a considerable fraction of sex difference in CHD risk. Higher levels of risk factors were associated with an age-related increase in the incidence of CHD and death in the two genders, yet in women more so.