Several studies have emphasized the substantial medicinal properties of clary sage (scientifically: Salvia sclarea) oils or extracts in preventing and treating a wide variety of health issues and disorders. GC-Mass investigated the chemical components of sage oil. Examining the free radicals scavenging potency of the oil was performed via assay of DPPH, for the biological effect of sage oil on the development and progression of cancer cells. The oil's antiproliferative capacity on cancer cells (toxicology test) was assessed on the hepatocellular carcinoma (HCAM) cell line using the MTT viability test. According to the results of the GS-MS analysis, the procedure successfully yielded 100% total identification of the chemical composition of Clary sage oil, of those are Gamma-terpinene (17.847%), heptane (16.759%), D-Limonene (12.654%), isopropyl myristate (10.179%), and linalool (8.189%). The examined antioxidant activity showed an increase in the level of catalase enzyme at 1.5h followed by complete reduction at 72h post-treatment. SOD was also reduced significantly after 72 h incubation and was not observed to be affected by exposure to oil. The cytotoxic activity of the oil was evident at (IC50=17.32µg/ml) when compared to the control, and the genomic DNA integrity of the HCAM cell line was degraded as presented on a gel. These findings indicate the potential use of Clary sage oil as an antioxidant and anti-cancer agent. The current investigation highlights the importance of setting up shorter exposure intervals with the sage oil to achieve the best findings concerning the pathophysiological cues against hepatocellular carcinoma progression.