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Isolation and Diagnosis of Some Types of Fungi Using PCR Technology for Infection by (Musca domestica L.) From some Areas of Anbar Governorate

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Last updated: 04 Jan 2025

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Abstract

This study revealed the presence of several fungal species, some of which are saprophytic, and others are pathogenic, in addition to the presence of fungi used in biocontrol in soil samples collected from six different regions of Anbar province. The identified fungal genera include Metarhizium anisopliae, Trichoderma harzianum, Penicillium, Fusarium spp., Rhodotorula, Rhizopus, Candida spp., and Aspergillus flavus. Confirmation of the diagnosis of the pathogen of the housefly (Musca domestica) using PCR technique indicated the presence of different genera of fungi at various concentrations, with the highest frequency observed for Aspergillus flavus (78.2%) and the lowest frequency for Trichoderma harzianum (6.5%). Among the isolated fungi, pathogenic fungi belonging to the genus Metarhizium anisopliae and Trichoderma harzianum were identified. Molecular diagnosis of the two pathogenic fungi was conducted using the polymerase chain reaction (PCR) technique, and the purity of the isolates ranged between 1.69 and 1.94, with concentrations ranging from 34.03 to 52.10 ng/ml. The newly isolated strains were deposited in the NCBI Gene Bank under the researchers' names.

DOI

10.21608/jbaar.2024.285734.1045

Keywords

Diagnosis of some types of fungi, PCR molecular diagnostic technique, Trichoderma harzianum, Metarhizium anisopliae

Authors

First Name

Saffanah

Last Name

Hardan

MiddleName

Mushrif

Affiliation

Anbar

Email

aabdulbaset2@gmail.com

City

Ramadi

Orcid

-

First Name

Thamer

Last Name

Muhsen

MiddleName

A. A.

Affiliation

Dept of Biology, College of Education for pure Science (Ibn Al-Haitham), University of Baghdad, Iraq.

Email

thamer.a.m@ihcoedu.uobaghdad.edu.iq

City

Baghdad

Orcid

0000-0002-5025-9278

Volume

10

Article Issue

2

Related Issue

47561

Issue Date

2024-06-01

Receive Date

2024-04-28

Publish Date

2024-06-01

Page Start

276

Page End

289

Print ISSN

2356-9174

Online ISSN

2356-9182

Article File

JBAAR_Volume 10_Issue 2_Pages 276-289.pdf

PDF . 2.3MB

Link

https://jbaar.journals.ekb.eg/article_361804.html

Detail API

https://jbaar.journals.ekb.eg/service?article_code=361804

Order

361,804

Type

Original Article

Type Code

1,272

Publication Type

Journal

Publication Title

Journal of Bioscience and Applied Research

Publication Link

https://jbaar.journals.ekb.eg/

MainTitle

Isolation and Diagnosis of Some Types of Fungi Using PCR Technology for Infection by (Musca domestica L.) From some Areas of Anbar Governorate

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Type

Article

Created At

25 Dec 2024