Because the Ehrlich ascites carcinoma (EAC) model can accurately predict survival time and is successful in creating neoplastic cells, it is frequently employed in experimental cancer studies. The present work is designed to investigate the antitumoral properties and therapeutic potential of Chia (Salvia hispanica) seed extract (ChSE) in inhibition of EAC-induced liver toxicity and tissue injury. All of the Forty female Swiss albino mice were equally separated into four cohorts (Gp1, control group; Gp2, CHSE group; Gp3, EAC group; Gp4, EAC+ChSE). Current results revealed that; EAC caused a notable increase in serum Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) activities, body weight, liver weight, liver injury, and a significant depletion in albumin and total proteins as in contrast to the control Gp. Post-treatment of EAC with CHSE (EAC+ChSE) induced a significant depletion in serum ALT, AST, ALP, body weight, liver weight, liver injury, and a notable rise in albumin and total proteins as compared to EAC. One may conclude that ChSE possesses the capacity for a liver protective effect against EAC cell-induced liver injury and signposts about the potential benefits of ChSE in the treatment of liver toxicity.