Probiotics play a crucial role in fighting infectious diseases by significantly impacting their control. They possess the capacity to lower the frequency of contagious illnesses, potentially lessening the dependence on antibiotics and helping to alleviate the development of bacteria that are resistant to various medications. Besides, they hold substantial importance in the realm of food. The objective of this research was to isolate various strains of lactic acid bacteria (LAB) from diverse sources and explore their capabilities in laboratory settings. The efficacy of probiotic strains against some harmful bacteria was assessed through both the agar diffusion method and the agar spot method. Additionally, their resilience under conditions resembling the stomach and intestinal environment, their capacity for auto-aggregation, and antibiofilm were also examined. Our findings suggest that the isolated strains displayed differing levels of probiotic effectiveness, with the HR3 strain, identified as Lactiplantibacillus plantarum, demonstrating notable excellence in this regard.