This paper presents the Physicochemical effects of organic solvents on a painted layer during the cleaning process in the conservation of mural painting. An experimental study was carried out replicating mural paintings followed by using two organic solvents (Ethanol and Benzene), which were applied on Egg yolk binder mixed with Malachite. This experiment was performed by a range of complementary analytical techniques used to examine treated samples, such as FTIR (Fourier- Transform Infrared Spectroscopy) analysis, Colorimetric Measurements and the Scanning Electron Microscope (SEM). The Surface Morphology analysis by SEM confirms an increase in the roughness with a fairly even surface topography at the micro level as the grains of pigment becomes more evident and more light scattering than a saturated paint film. The FTIR analysis indicated that the amides of the secondary structure of proteins were affected by organic solvents. Colorimetric measurements showed a total color difference ∆E in the sample with benzene.