Medicinal plants play a vital role in traditional folklore, as they can prevent and treat many diseases caused by microbes. In the present study, the antibacterial activities of ethanolic extracts of two medicinal plants Neem plant (Azadirachta indicia) and Senna alexandrina were evaluated against Gram-positive bacteria Staphylococcus aureus, Enterococcus faecalis, and MRSA. Neem (Azadirachta indica) leaves ethanolic extracts produced significant antibacterial potential against Gram-positive bacteria. For Enterococcus faecalis at different concentrations of Neem ethanolic extracts (400, 300, 200, and 100 mg/ml) it gives different inhibition zones 12, 11, 10, and 5mm respectively, and for Staphylococcus aureus it gives different inhibition zones 13,11,9 and 3mm respectively, and against MRSA, it gives 12,11,9 and 0 mm respectively. MIC of Neem (Azadirachta indica) leaves ethanolic extracts against Enterococcus faecalis was at 150 mg/ml, MIC against Staphylococcus aureus was at 9.3 mg/ml, and for MRSA MIC was at 37.5mg/ml, but the antibacterial activity of S. alexandrina plant extract at different concentrations starting from (400,300,200 and 100 mg/ml) It has shown a great antibacterial potential against Staphylococcus aureus it gives different inhibition zones 22, 20, 18 and 13mm respectively. But it has shown no effect on Gram-positive bacteria Enterococcus faecalis and MRSA, since it did not give any growth inhibition zone with these bacteria.