Stem cells research and associated therapies are advancing remarkably. The potential to separate stem cells from menstrual blood has created a new avenue in stem cells research that may one day provide mankind with therapeutic advantages. Aim: The current study aimed to examine the effect of jigsaw teaching strategy on internship nursing students' health literacy regarding menstrual blood banking.  Methods: A quasi-experimental design (one-group pretest-posttest) was adopted. A convenience sample of 100 internship nursing students were recruited. Data were collected using two tools: structured self-administered questionnaire and menstrual blood banking attitude assessment scale. Results:  Study findings revealed that, pre-intervention the mean knowledge score was 7.70±2.40 while at post-intervention it was significantly increased to 12.2±2.02 (p= 0.02). Concerning attitude, only 6.0% of students expressed positive attitude regarding menstrual blood banking pre-intervention as compared to 78.0% of them post-intervention (p<0.001). The current study declared that, there was a statistically significant correlation between knowledge and attitude at post-intervention (p =0.003). Conclusion: jigsaw teaching strategy had a significant positive impact on internship nursing students' health literacy towards menstrual blood banking. Recommendation: Utilization of such teaching strategy should be encouraged to improve health literacy among internship nursing students.