Background: Workplace incivility has been noted as a pervasive and serious problem in the healthcare sector that may have a negative impact on staff nurses and organizational outcomes, such as turnover intention. The study aimed to explore the effect of workplace incivility on the turnover intention of working staff nurses at a selected hospital. Method: Design: A descriptive‑correlational research design was utilized. Setting: This study was conducted at Cairo University Hospitals including different units. Subjects: A convenience sample of 250 staff nurses who were working in different units at Cairo University Hospitals. Tools: two tools were used as follows: (I) Nurses' Perception of Workplace Incivility Questionnaire and (II) Nurses' Turnover Intention Questionnaire. Results: The findings showed that 66% of staff nurses had a high perception level of workplace incivility and 67% of them had a high turnover intention level. Conclusions: there was a highly significant statistical correlation between nurses' total perception of workplace incivility and their turnover intention, also there was a highly significant statistical positive effect of workplace incivility on nurses' turnover intention level. Recommendations: Nursing managers should develop restricted policies with clear procedures for tracking, preventing, and disciplining staff incivility behaviors. Nursing managers should carefully detect the contributing factors of staff turnover intention and take corrective actions to enhance staff satisfaction.