Background: Blood transfusion is considered a highly successful and potentially life-saving treatment, especially for pediatric patients. However, ensuring effective and safe administration of blood transfusions requires proper education and training for pediatric nursing staff. Aim: This study aimed to examine the effectiveness of implementing a training module about blood transfusion on pediatric nurses' competence. Design: The study utilized a quasi-experimental approach, employing a one-group pre- and post-test design. Settings: The research was conducted at five departments within the Mansoura University Children's Hospital, including hematology, pediatric intensive care unit, emergency, surgical, and medical departments. Subjects: A convenient sample of 80 pediatric staff nurses was included in the current study. Tools: Data were collected using a structured interview questionnaire to assess nurses' knowledge levels and a blood transfusion competency checklist to evaluate their practices before, during, and after the training module. Results: The results showed a highly statistically significant difference in the mean scores of nurses' knowledge levels and practice regarding blood transfusion before and immediately after the training intervention (P<0.001**). Conclusion: The application of a blood transfusion training module for pediatric nurses improved their knowledge regarding blood transfusion. Furthermore, they demonstrated improvement in practical skills, leading to a higher practice score than before the training. Recommendation: The study recommended organizing regular training programs, using simulation-based learning, encouraging continuing education, facilitating collaboration and peer learning, standardizing procedures, fostering a safety culture, promoting multidisciplinary collaboration, and engaging in quality improvement initiatives.