Recently, there are increasing numbers of unmarried healthy reproductive-aged females and young females who have diseases associated with early loss of ovarian reserve. Oocyte cryopreservation is a specific technique for freezing one or more unfertilized ovum to be used in the future to get pregnancy. Aim: The aim of this study was to determine effect of the educational program on knowledge, attitudes, and intention of unmarried healthy females regarding oocyte cryopreservation. Subjects and method: Design: A quasi experimental research design was used to conduct this study. Setting: The study was conducted at six faculties of Tanta University Tanta Egypt. Subjects: A simple random sample of 100 unmarried working females was included in the study. Tools: (I): Knowledge, (II): Attitudes, and (III): Intention of unmarried females regarding oocyte cryopreservation. Results: There was a highly statistical significant difference between pre and post-program implementation in the studied unmarried healthy females' knowledge, attitudes, and intention regarding oocyte cryopreservation (p<0.001*). Conclusion: Implementation of the educational program improved knowledge, attitudes, and intention of the studied healthy unmarried females regarding oocyte cryopreservation. Recommendations: Heath education and counselling programs, modules, and guidelines regarding oocyte cryopreservation should be provided by maternity and gynecological nurses to unmarried healthy females to help them have an informed decision to preserve their fertility in order to be pregnant during their preferred time.