Aim: The purpose of this study is to compare the efficacy of of Bupivacaine 0.25% alone versus the Bupivacaine 0.25% with Hyaluronidase 1500 I.U during ultrasound guided transversus abdominis plane block in elective laparoscopic cholecystectomy under general anesthesia. Patients and methods: This Randomized Controlled-double-blind study was conducted on 60 Patients randomized into two groups. Group A (30 patients), TAP block was performed using Bupivacaine 0.25% 20 ml on each side of abdominal wall. Group B (30 patients), TAP block was performed using a combination of 18 ml of Bupivacaine 0.25% and 2 ml of Hyaluronidase 750 I.U on each side of abdominal wall. This study was carried out in the department of Anesthesiology, surgical intensive care and pain management in Beni-Suef University hospital. Results: Regarding the total analgesic amount requirements, this study showed that there was no statistically significant difference between both groups regarding the dose of analgesia after 2, 4, 6, 12, 16, 20, and 24 postoperatively. There was a statistically significant longer mean time to 1^st request of analgesia being 10.2±3.1 hours in bupivacaine and hyaluronidase group than bupivacaine only group being 5.2±1.9 hours. According to the NSR pain score, there was no statistically significant difference between bupivacaine and hyaluronidase group and bupivacaine only group after 2, 4, 6, 8, 12, 16, 20, and 24 of the operation. Conclusion: Using hyaluronidase as an adjuvant to bupivacaine in sonar-guided TAP block after laparoscopic cholecystectomy offered good postoperative analgesia, postponed the need for analgesia, and decreased analgesic intake early postoperatively.