Background: Diabetic nephropathy is a chronic disorder that takes years to develop and is characterized by a progressive rise in urinary albumin secretion. It is one of the primary consequences of diabetes mellitus. Aim and objectives: To investigate the relation between kidney injury molecule1 (KIM1) and diabetic nephropathy. Patients and methods: This was a case control research performed on 100 subjects at internal medicine department of beni-suef university hospital. Thecases were separated into 4 groups. Results: KIM -1 showed  statistically significant varianceamongst the four studied groups with P value ( <0.001) ;In Control group KIM-1 varied from 1.70 to 27.50 with mean ± SD = 12.21 ± 7.249 , In normo-albuminuric group  KIM-1 ranged from 21 to 36.90 with mean  ± SD = 27.11 ± 4.48 ,  in micro-albuminuric group KIM-1 ranged from 10.1 to 53 with mean ± SD= 42.8 ± 9.33,inmacro- albuminuric group KIM-1 ranged from.69.to 81.5 with mean ± SD = 75.5 ± 4.03. Conclusion:Our study revealed increase of serum KIM 1 levels with development of Diabetic Nephropathy, reflecting the important role of Kim-1-as a biomarker for early diagnosis-and progression of DN. Serum Kim-1 is recommended to be utilized as a warning system for the development of diabetic nephropathy in people with diabetes as a screening test.