Background: Klebsiella pneumoniae is one of the common causes of nosocomial infection. Antibiotic resistance in addition to the capability of forming biofilm, as 2 essential virulence factors of K. pneumoniae, are responsible for the persistent infections. The aim of the present study was to assess the correlation between antimicrobial resistance and the ability to form biofilm in K. pneumoniae strains isolated from hospitalized cases at urology department with their tendency to form biofilm.  Methods: Over a 6-month duration, a total of 25 K. pneumonia isolates were collected. Antibiotic susceptibility was detected using Kirby–Bauer disk diffusion approach based on CLSI. Biofilm formation was evaluated using tissue culture plate method. Lastly, isolates were tested for ESBL production. Results: All the identified klebsiella isolates (100%) showed resistance to cefepime, 92 percent of the isolates showed resistance to ceftazidime, 80% showed resistance to both cefotaxime and ceftriaxone and 72% were resistant to  ciprofloxacin, 28% showed multidrug resistance, 20% were extensive drug resistant and 8% were pan drug resistant. The results of biofilm formation in the tissue culture plate assay indicated that 15 (60 %) strains might produce biofilm and only 10 (40 %) isolates weren't capable of forming biofilm. Conclusion: The current study findings supported the importance of biofilm formation in resistance to antimicrobial agents.Further studies concerning the mechanism of biofilm production in K. pneumoniae may finally help treating biofilm-mediated infections.