Even though some polyherbal combinations have been used traditionally, there is limited scientific research on their safety and effectiveness. To address this gap, this study set out to explore the potential synergies and safety of a polyherbal formulation that contains A. monosperma and M. piperita. Specifically, the study examined the impact of this formulation on certain biochemical and hematological parameters in vivo. Materials and Methods: The study involved organizing the animals into eight groups, each comprising five animals. Group1, served as control group received distilled. Groups 2, 3, and 4, referred to as AL, AM, and AH received A. monosperma in doses of 1000, 3000, and 5000 mg/kg body, respectively. Group5, received 290 mg/kg of M. piperita extract. The remaining three groups, ALM, AMM, and AHM, received a mixture of A. monosperma and M. piperita in a polyherbal formulation (PF) at the same doses for 14 days, once daily. Results: The results showed that none of the treated animals died or showed signs of toxicity. Additionally, the polyherbal formulation did not cause any notable shifts in body weight or blood-related parameters, when compared to the control groups. Furthermore, the hepatic and renal functions remained unaffected using this formulation. Conclusion: Therefore, it can be concluded that the polyherbal formulation is harmless and non-poisonous, even at high doses of 5000 mg/kg, and has the potential to be employed as a diabetes remedy.