The field experiment was carried out at agriculture research station, Al-Arish, North Sinai, Egypt during two growing seasons(2020/2021 and 2021/ 2022).Three Vicia faba L. cultivars (Sakha2, Nubarya1 and Maryot2) were evaluated under water stress levels (severe stress (50%), moderate stress (75%) and control treatment (normal irrigation) (100%) from evapotranspiration to study tolerance indices and biochemical traits. Results indicated that seed yield increased by increasing the water level. The higher level of irrigation the more efficient of crop yield. The reduction in yield/feddan was in linear relation with the increase of water deficit stress. Maryot2 cultivar exceeded the other cultivars in number of pods and seed yield. In contrast, Sakha2 achieved the lowest values for each of these traits in the same conditions while it recorded the highest values in 100 seed weight trait. The interaction effect of water deficit stress and cultivar was significant on seed yield of the faba bean. Water deficit stress significantly reduced seed yield for the three cultivars. Stress tolerance indices classified Maryot2 as the most relatively tolerant cultivar based on drought tolerance index (DTI) in terms of seed yield/feddan valued (0.87 and 0.88) in the 1^st and 2^nd seasons, respectively, while Sakha2 cultivar recorded the lowest relative tolerance values (0.75 and 0.75) during the growing seasons. Based on yield injury (%), Maryot2 cultivar recorded the highest decrease in seed yield (21.51) in the first season while Nubarya1 recorded the highest value of yield injury in the second season (21.10). In contrast, Sakha2 was the least affected. The protein profile under water deficit stress demonstrated that there were differences among cultivars in response to water deficit stress. Few numbers of new bands were observed only in water deficit stress treated plants for each cultivar and few numbers of bands were disappeared in treated plants compared with control treatment for each cultivar.