A field experiment was conducted at a private farm in Al-Qassasin City, Ismailia Governorate, Egypt, during two successive seasons 2018/2019 and 2019/2020 to assess the impact of seed inoculation with nitrogen-fixing bacteria on the growth, root, sugar yields, and quality of different sugar beet varieties (Cleopatra, Senderla, and Capel) in sandy soil. There has been a recent increase in interest in bioorganic fertilizers as means to mitigate the negative effects of intensive farming practices. N-fixing microorganisms have an influence on plant nutrition and also play a crucial role in crop biofertilization as rhizobacteria that promote plant growth. The experimental design utilized a randomized complete block with three replication sub-plots. All treatments in the current study (inoculation of N-fixing bacteria) resulted in increased yield and its components compared to the untreated controls. The treatment of 100kgN/fad+900g Cerialine produced the best root yield of 43.91 ton/fad and the highest sugar yield of 5.31ton/fad in the second season. The highest root fresh weight of 996.5 g/plant was obtained at 210 days with Senderla in the first season. Cleopatra with 100kgN/fed+900g Cerialine had the highest root and sugar yield of 44.86 and 5.87 ton/fad, respectively in the second season. In comparison to the control, inoculating sugar beet seeds with 100 kg N/fad and 900 gm Cerialinee significantly increased sugar beet growth, quality, and productivity.