The present work was carried out at the apiary of honeybee research center, Faculty of Environmental Agricultural Sciences, El-Arish University, Egypt during August - October of 2020 to evaluate the efficacy of honeybee venom at different concentrations (0.1, 0.01 and 0.001g/ 1L sucrose syrup) compared with a commercial acaricide, fluvalinate in controlling varroa mites in honeybee colonies. Moreover, the effects of these treatments on brood rearing activity were observed. Obtained results showed that weekly level of fallen Varroa on hive bottom board was high (average 92.7 mites/ colony) after using fluvalinate strips compared to bee venom averaged60.3, 46 and 28.3 mites/colony for 0.1,0.01 and 0.001concentrations, respectively. While the untreated colonies(control) averaged 7.3 mites/colony. Level of worker brood rearing was high in colonies treated with 0.1g bee venom, averaged 5441, 5857 and 6215 sealed cells/colony in the 2^nd, 3^rd and 4^th brood cycles (every 12 days) intervals, respectively compared to other venom treatments. However, the least number of sealed worker brood (3607 cells/ colony) was recorded in the 4^th interval influvalinate treatment.