ABSTRACT In the current study, silver nanoparticles (AgNPs) were biosynthesised using Dates seeds (Phoenix dactylifera L) aqueous extract (LAE). HPLC analysis was used to identify the chemical components, flavonoids and phenolics, using both descriptive and quantitative analytics. Transmission Electron Microscopy (TEM), Energy Dispersive X-Ray (EDX), Zeta Potential, and FT-IR analysis were used to characterize the generated powder of  NPs. The chemicals that were most frequently identified in the Dates seeds (Phoenix dactylifera L) .Extract by HPLC analysis were gallic acid, chlorogenic acid, pyrocatechol, and methyl gallate. The AgNPs were spherical in form and ranged in diameter from 8.70 nm to 13.4 nm. AgNPs have charged potentials of -10.1 according to zeta potential. FTIR spectra were used to characterize the hydroxyl functional group found in alcohols and phenolic substances. This demonstrated that using dates seeds (Phoenix dactylifera L)   extract from the Siwa region of Egypt to create green silver nanoparticles was successful.