ABSTRACT A field experiment was conducted to study the effect of two sowing dates ( 15 September and 15 October ) on root and sugar yield of five sugar beet varieties (Melodia , Fantazia , Gambol , Jamika and Javlen)  at three locations ( Ismailia , Nubaria and Benisuef ) during 2020/2021 and 2021/2022 seasons. The experiments were carried out in split plot design with three replications. The obtained results indicated that Nubaria location exceeded Ismailia and Benisuef in root and sugar yield. Sowing plants at 15 September gave higher yields of root and sugar per feddan than sowing at 15 October. Sugar beet varieties significantly differed in root and sugar yield per feddan in both seasons. The interaction between locations, sowing dates and varieties was significant on root and sugar yield in both seasons.  Sowing sugar beet variety Javlen at 15 September in Ismailia location gave the highest sugar yield, but at Nubaria location sowing sugar beet variety Melodia  at 15 September gave the highest sugar yield  in both seasons . While, at Benisuef location sowing sugar beet variety Gambol at 15 October gave the highest sugar yield as compared with all other this interaction treatments in both seasons.