ABSTRACT  A field experiment was conducted during two successive winter growing seasons of (2021/ 2022 and 2022/ 2023) at the experimental farm of Mallawi Agriculture Research Station, Minia Governorate, Egypt. The objective of this study was to illustrate the integrated effect of the mineral nitrogen fertilization and stimulant substances on wheat plants. A split-plot design was used, the main plot was stimulant substances (without, amino acids, seaweed extract and mix of amino acids plus seaweed extract). While the nitrogen fertilization (60%, 80% and 100% from recommended dose (RD), were assigned in sub-plot. The results showed that in most traits there was a significant affect interaction between stimulants and N fertilizer treatments. The highest grain yield (9.490, 9.455 ton ha^-1) was obtained by treating wheat with combination of amino acids plus seaweed extract with 80% of  Nitrogen Recommended Dose NRD  in both seasons of study, respectively. In addition, the increase in mineral contents and protein contents of wheat grain through the same treatment.