ABSTRACT A Field experiments was carried out in three sowing dates at two locations under conditions El-Mattana Research Station (latitude of 25.25°N and longitude of 32.31°E and elevation of 81 m above sea level) Luxor Governorate, And Kom Ombo Agricultural Research Station, (latitude of 24.28°N and longitude of 32.57°E and elevation of 84.5 m above sea level) Aswan Governorate, Egypt, during 2018/2019 and 2019/2020 seasons. To Evaluate of nine sugar beet varieties to bridge the sugar gap between production and consumption by expanding sugar beet cultivation in Upper Egypt. The present work included nine sugar beet varieties (Beta Vulgaris, L.) namely Cleopatra, Tarbelli, Betamax, Sirona, Capel, Saucona, FD17B410, FD18B418, and LP 17B411, to evaluate them And select the best in terms of suitability to environmental conditions and the extent of their superiority in yield and Quality . Sugar beet varieties were sawn in three dates (mid of September, October and November ±3) days between the two sites (El-Mattana and Kom Ombo) in the two planting seasons. The experiment was carried out in randomized strip plot design with three replications.   Results showed that El-Mattana location surpassed in the highest values root fresh weight, root length, root diameter, root yield (t/fed), sucrose %, Extractable sugar, purity, and sugar yield (t/fed) and less content of potassium percentages compared with Kom Ombo location. The results indicated that time of cultivation significantly affected each of average of root fresh weight, top fresh weight, root length, root diameter, root yield (t/fed), sucrose %, Extractable sugar%, purity%, and sugar yield (t/fed) and less content of impurities in month October. Examined Sugar beet varieties significantly differed in some studied traits in the two seasons. recorded Capel, Sirona and Beta max varieties recorded the highest values of growth, Quality and yield of Sugar beet compared to the other varieties a spatially sown in October month either El-Mattana or Kom Ombo location