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353603

Predominance of <i>bla</i>KPC carbapenemase gene in multi-drug resistant <i>Pseudomonas aeruginosa</i> isolated from ICUs and surgical wards in Egypt

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Antimicrobial resistance

Abstract

Background:  The last-resort classes of antibiotics with the best success in treating severe Pseudomonas aeruginosa (P. aeruginosa) infections caused by drug-resistant P. aeruginosa are carbapenems. So, we aimed to assess the frequency of carbapenem-resistant genes in P. aeruginosa. Methods: Samples isolated from critically ill patients at Intensive Care Units (ICUs) and surgical wards in Minia, Egypt were examined for imipenem-resistance phenotypically by Modified Hodge test (MHT) and Modified carbapenem inactivation method (mCIM) test and genotypically by PCR. Results: Fifty isolates (64%) of P. aeruginosa were resistant to carbapenems (imipenem and meropenem), of which: 16 (32%) were (MHT) positive and 26 (52 %) were (mCIM)-positive. Regarding resistance genes, blaIMP, blaVIM, and blaKPC were detected in 14%, 20%, and 48% of the carbapenem resistant Pseudomonas aeruginosa (CRPA) isolates; respectively, while blaGIM, blaSIM, blaNDM-1 and blaSPM-1 were not detected.  Conclusion: There is a high prevalence of multi-drug resistant (MDR) strains of P. aeruginosa. Doctors should pay attention to carbapenem resistance. mCIM test was much more sensitive than MHT for phenotypic detection of CRPA isolates. blaKPC gene was the most frequently detected gene.

DOI

10.21608/mid.2024.279851.1883

Keywords

Keywords: <i>P. aeruginosa</i>, mCIM, MHT, carbapenemases, PCR

Authors

First Name

Noha

Last Name

Hassuna

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, Egypt

Email

nohaanwar@mu.edu.eg

City

Minia

Orcid

0000-0001-6702-2341

First Name

Nehal

Last Name

Koriem

MiddleName

E.

Affiliation

Botany and Microbiology, Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt

Email

nehalessam65@yahoo.com

City

Minia

Orcid

-

First Name

Shaimaa

Last Name

Zaki

MiddleName

Hassouna

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, Egypt

Email

shimaa.minia@gmail.com

City

Minia

Orcid

-

First Name

Medhat

Last Name

Abd El Fattah

MiddleName

-

Affiliation

Botany and Microbiology, Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt

Email

drmfatahme@gmail.com

City

Minia

Orcid

-

First Name

Amani

Last Name

Abdelmoaty

MiddleName

Mohammed

Affiliation

Pharmaceutics, Faculty of Pharmacy, Beni-Suef University, Beni-Suef University, Beni Suef, Egypt

Email

amani_mcc@hotmail.com

City

Minia

Orcid

-

First Name

Reham

Last Name

Ibrahem

MiddleName

A.

Affiliation

Microbiology and Immunology Department, Faculty of Pharmacy, Minia University, Minia, Egypt.

Email

rehamee_micro@yahoo.com

City

Minia

Orcid

-

Volume

5

Article Issue

3

Related Issue

49567

Issue Date

2024-08-01

Receive Date

2024-04-06

Publish Date

2024-08-01

Page Start

1,127

Page End

1,138

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 5_Issue 3_Pages 1127-1138.pdf

PDF . 865.7kB

Link

https://mid.journals.ekb.eg/article_353603.html

Detail API

https://mid.journals.ekb.eg/service?article_code=353603

Order

23

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Predominance of <i>bla</i>KPC carbapenemase gene in multi-drug resistant <i>Pseudomonas aeruginosa</i> isolated from ICUs and surgical wards in Egypt

Details

Type

Article

Created At

25 Dec 2024