Background:   Leishmaniasis produced by flagellated protozoa return to genus Leishmania. The parasites are transmitted from human to human by bite of Phledotamine sand flies. Objectives: The current study is aimed to detect prevalence of Leishmania tropica among Sudanese patients. Methods: This study conducted in dermatology hospitals in Khartoum Province (Army forces hospital, Khartoum and Omdurman teaching hospitals). Sixty positive cases are obtained by repeated direct smear that were examined by PCR techniques. DB8 and AJS3 primers is used to amplify Cytochrome Oxidase Ⅱ gene for identifying leishmania parasite. Results: Regarding to the gender, the frequency of the infection in the male is far higher (96.7%) than in females (3.3%),14(23.3%) among the accountants, students and others occupations respectively. According to duration of the infection 4(6.7%) 6(10%),10(16.7%),2(3.3%) ,16(26.7),6(10%),7(11.7%),9(15%) got the infection 1,2,3,4,5,6,10 months and one year before starting this study respectively. Considering occupation, the soldiers 36(60%) were more frequently affected by Leishmania tropica complex, while the distribution was 7(11.7 %,) 3(5%) ,14(23.3%) among the accountants, students and others occupations respectively. Conclusions:  The Cytochrome Oxidase Ⅱ is suitable for determining cutaneous leishmaniasis parasite, which is more common gene in KDNA. Further research is needed with more large sample size.