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Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier

Article

Last updated: 04 Jan 2025

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Subjects

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Tags

Antimicrobial resistance

Abstract

Background: The production of metallo-beta-lactamases (MBL) such as VIM (Verona integron-encoded metallo-β-lactamase), IMP (Imipenem-resistant Pseudomonas) and NDM (New Delhi metallo-β-lactamase) in Gram-negative bacilli (GNB) resistant to carbapenems is a real concern for clinicians, given the therapeutic impasses involved. However, the existence of resistance genes encoding these enzymes is virtually undocumented in Burkina Faso. Aim: To genotypically demonstrate carbapenem resistance through the production of VIM-type carbapenemases in GNB strains collected at the Centre Hospitalier Universitaire de Tengandogo (CHU-T) and the Hôpital Saint Camille de Ouagadougou (HOSCO) in Burkina Faso. Methods: In this study, the resistance profile of 158 strains of GNB to imipenem, meropenem, ertapenem, doripenem and aztreonam was determined using the disc diffusion method. Resistant strains were analyzed by conventional PCR to detect blaVIM using specific primers. Results: Of 158 GNB strains collected, 91 (57.6%) were resistant to at least one of the carbapenems and/or aztreonam. The highest prevalence of resistant strains was observed in Escherichia coli (E. coli) 45.1% (n=41) and Klebsiella pneumoniae (K. pneumonia) 26.5% (n=24), which are the majority species. The blaVIM gene was detected in only 7 resistant strains (7.7%), including 3.3% (n=3/91) of E. coli, and 1.1% (n=1/91) of each of the species Pseudomonas aeruginosa (P. aeruginosa), Klebsiella oxytoca (K. oxytoca), Proteus mirabilis (P. mirabilis) and Serratia marcescens (S. marcescens). Conclusion: This study established the existence of blaVIM gene, which is involved in the resistance of GNB to carbapenems through the production of VIM-type enzymes at CHU-T and HOSCO in Burkina Faso.

DOI

10.21608/mid.2024.262512.1760

Keywords

Carbapenem resistance, Carbapenemase, blaVIM, Gram-negative bacilli, Burkina Faso

Authors

First Name

Damis Yves Patrik

Last Name

BOUNIOUNOU

MiddleName

-

Affiliation

Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso

Email

patrikbiochsnp4@gmail.com

City

-

Orcid

-

First Name

Amana

Last Name

Metuor Dabiré

MiddleName

-

Affiliation

Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso , Training and Research Unit in Applied Sciences and Technologies (UFR/SAT), University of Dedougou, Burkina Faso

Email

ametuordabire@yahoo.fr

City

Ouagadougou

Orcid

-

First Name

Nicolas

Last Name

OUEDRAOGO

MiddleName

-

Affiliation

National Center for Research and Training on Malaria (CNRFP), National Institute of Public Health (INSP), Burkina Faso

Email

onicolas85@yahoo.fr

City

Ouagadougou

Orcid

-

First Name

Rahimatou Yasmine

Last Name

Tiemtore

MiddleName

-

Affiliation

Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso

Email

thiombianomina@yahoo.fr

City

Ouagadougou

Orcid

-

First Name

Serge

Last Name

Sougué

MiddleName

-

Affiliation

Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso , National Agency for Safety of the Environment, Food, Work and Health products (ANSSEAT), National Institute of Public Health (INSP), Burkina Faso

Email

sergesougue@yahoo.fr

City

Ouagadougou

Orcid

-

First Name

Pegdwendé Rose

Last Name

Bonkoungou

MiddleName

-

Affiliation

Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso Saint Thomas Aquinas University, Ouagadougou Burkina Faso

Email

rosebonkoungou05@gmail.com

City

Ouagadougou

Orcid

-

First Name

Jacques

Last Name

Simporé

MiddleName

-

Affiliation

Molecular Biology and Genetics Laboratory (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou, Burkina Faso , Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou, Burkina Faso

Email

jacques.simpore@yahoo.fr

City

Ouagadougou

Orcid

-

Volume

5

Article Issue

2

Related Issue

47401

Issue Date

2024-05-01

Receive Date

2024-01-14

Publish Date

2024-05-01

Page Start

763

Page End

773

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 5_Issue 2_Pages 763-773.pdf

PDF . 788.8kB

Link

https://mid.journals.ekb.eg/article_344552.html

Detail API

https://mid.journals.ekb.eg/service?article_code=344552

Order

34

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Multiresistance to carbapenems by production of Verona integron-encoded metallo-β-lactamase (VIM) -type carbapenemases in Gram-negative bacilli isolated at the Centre Hospitalier

Details

Type

Article

Created At

25 Dec 2024