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HPLC fingerprint profile, in-vitro cytotoxicity and anti-herpes simplex virus activity of methanol extract from <i>Strophanthus hispidus</i> DC (Stem bark).

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Pharmaceutical microbiology

Abstract

Herpes simplex virus (HSV) infection is quite common in adults and neonates and is associated with oral mucocutaneous lesions and/or genital infections. Therefore, the aim of this present study was to determine the in-vitro cytotoxicity and anti-herpes simplex virus (1 and 2) activity of methanol extract of Strophanthus hispidus (S. hispidus ) DC (Stem bark) and his HPLC fingerprint profile. The cytotoxicity test on Vero cells was performed by MTT assay and various concentrations of extract less than the maximum nontoxic concentration were used against Vero cells for anti-HSV-1 and ant-HSV-2 effects by plaque assay. The CC50 values of methanolic extracts of S. hispidus (stem bark) and acyclovir against Vero cells were 94.46 µg/mL and 118.78 µg/mL, respectively.  Whereas the EC50 and selectivity index values of this extract against herpes simplex virus 1 and herpes simplex virus 2 strains were 36.60 µg/mL (SI=2.58) and 39.47 µg/mL (SI=2.39), respectively. HPLC finger-printing of this extract was developed and showed seven major compounds including ascorbic acid, quercetin, resorcinol and gallic acid represented in large amount. In conclusion, the methanolic extract of Strophanthus hispidus DC(stem bark) has good antiherpetic activity against HSV- 1 and HSV-2 but it is also highly toxic. Therefore, it will be important to separate toxic molecules from those responsible of the antiherpetic activity by bio guided fractionation procedures.

DOI

10.21608/mid.2022.171059.1409

Keywords

Strophanthus hispidus DC, Cytotoxicity, anti-Herpes Simplex Virus type 1 and 2, antiviral activity, HPLC

Authors

First Name

Arnold

Last Name

Mulula

MiddleName

-

Affiliation

Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo)

Email

arnold.mulula@unikin.ac.cd

City

-

Orcid

0000-0003-3034-4181

First Name

Abdeldjalil

Last Name

Bouzina

MiddleName

-

Affiliation

Department of Botany and Microbiology, Faculty of Sciences, Cairo University, Egypt.

Email

bouzinaabdeldjalil96@gmail.com

City

Cairo

Orcid

-

First Name

Hugues

Last Name

Mambu

MiddleName

Bisi

Affiliation

Cliniques Universitaires de Kinshasa, University of Kinshasa, Congo.

Email

huguesmambubs@gmail.com

City

Kinshasa

Orcid

-

First Name

Joséphine

Last Name

Ntumba

MiddleName

Kankolongo

Affiliation

Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo)

Email

kankolongojosephine574@gmail.com

City

Kinshasa

Orcid

-

First Name

Joachim

Last Name

Nsomue

MiddleName

-

Affiliation

Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo)

Email

-

City

-

Orcid

-

First Name

Milka

Last Name

Tshingamb

MiddleName

Ndaya

Affiliation

Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo) Institut National de Recherches Biom&eacute;dicales (INRB) Democratic Republic of Congo

Email

milkatshingambndaya@gmail.com

City

Kinshasa

Orcid

-

First Name

K.T.

Last Name

Kashishi

MiddleName

-

Affiliation

Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo)

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Zaki

MiddleName

-

Affiliation

Department of Chemistry, Faculty of Sciences, Ainshams University, Egypt.

Email

ahmeddzakih@gmail.com

City

Cairo

Orcid

-

First Name

Taba

Last Name

Kalulu

MiddleName

-

Affiliation

Department of Chemistry, Faculty of Sciences and technologies, University of Kinshasa (Democratic Republic of Congo)

Email

kalulutaba@gmail.com

City

Kinshasa

Orcid

-

Volume

5

Article Issue

1

Related Issue

45807

Issue Date

2024-02-01

Receive Date

2022-10-26

Publish Date

2024-02-01

Page Start

379

Page End

388

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 5_Issue 1_Pages 379-388.pdf

PDF . 1.1MB

Link

https://mid.journals.ekb.eg/article_269412.html

Detail API

https://mid.journals.ekb.eg/service?article_code=269412

Order

39

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

HPLC fingerprint profile, in-vitro cytotoxicity and anti-herpes simplex virus activity of methanol extract from <i>Strophanthus hispidus</i> DC (Stem bark).

Details

Type

Article

Created At

25 Dec 2024