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320661

Exploring rapid molecular methods for diagnosing <i>Candida</i> species infecting humans: A narrative review.

Article

Last updated: 04 Jan 2025

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Tags

Clinical microbiology

Abstract

Background:  Candida species are perilous fungal pathogens that can cause various human infections. Accurate and timely identification of these fungi is crucial for appropriate treatment selection and effective disease management. Traditional methods for Candida species identification and characterization, such as phenotypic assays and culture-based techniques, have limitations in accuracy, time-consuming processes, and limited species differentiation. In recent years, molecular methods have emerged as powerful tools for rapid and accurate identification and characterization of Candida species. The molecular methods developed and employed for identifying and characterizing Candida species. It begins by highlighting the challenges associated with conventional methods and the need for more efficient and reliable techniques. The abstract then explores various molecular approaches, including polymerase chain reaction (PCR) assays, DNA sequencing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). These methods leverage the genetic and proteomic characteristics of Candida species to provide accurate and specific identification and strain differentiation. Furthermore, this article discusses the application of molecular methods in clinical settings, epidemiological studies, and antifungal resistance monitoring. Molecular techniques enable rapid detection of Candida species directly from clinical samples, facilitating early diagnosis and prompt initiation of appropriate antifungal therapy. They also play a crucial role in epidemiological investigations, allowing for the identification of clonal outbreaks and the tracking of transmission patterns. Additionally, molecular methods aid in detecting genetic markers associated with antifungal resistance, enabling tailored therapeutic approaches.

DOI

10.21608/mid.2023.239051.1624

Keywords

Candida species, Infections, Molecular diagnosis, Antifungal therapy

Authors

First Name

Debasmita

Last Name

Dubey

MiddleName

-

Affiliation

Medical Research Laboratory, IMS and SUM Hospital, Siksha O Anusandhan (deemed to be) University, Bhubaneswar, Odisha

Email

debasmitadubey@gmail.com

City

Bhubaneswar

Orcid

-

First Name

Shakti

Last Name

Rath

MiddleName

-

Affiliation

Central Research Laboratory, Institute of Dental Sciences, Siksha O Anusandhan Deemed to be University, Bhubaneswar, Odisha

Email

dr.shaktirath@gmail.com

City

Bhubaneswar

Orcid

-

First Name

Sushree

Last Name

Subhadarshini

MiddleName

Swagatika

Affiliation

Department of Paramedics and Allied Sciences, Centurion University of Technology and Management, Bhubaneswar, Odisha, India

Email

sushreeswagatika@gmail.com

City

Bhubaneswar

Orcid

-

First Name

Gopal

Last Name

Purohit

MiddleName

Krishna

Affiliation

Director, Heredity Biosciences, Bhubaneswar. Odisha, India`

Email

gopalpurohit@hereditybio.in

City

Bhubaneswar

Orcid

-

First Name

Debasish

Last Name

Tripathy

MiddleName

-

Affiliation

Research Assistant,Heredity Biosciences and Research, Jayadev Vihar, Bhubaneswar, Bhubaneswar, Odisha, India

Email

debasishtripathy@gmail.com

City

Bhubaneswar

Orcid

-

First Name

Rajashree

Last Name

Panigrahi

MiddleName

-

Affiliation

Department of Microbiology,IMS and SUM Hospital, Siksha O Anusandhan (deemed to be) University, Bhubaneswar, Odisha

Email

rajashreepanigrahi@soa.ac.in

City

Bhubaneswar

Orcid

-

First Name

Sourav

Last Name

Palai

MiddleName

-

Affiliation

Research Assistant, Central Research Laboratory, Institute of Dental Sciences, Siksha O Anusandhan (deemed to be) University, Bhubaneswar, Odisha, India

Email

souravsrv504@gmail.com

City

Bhubaneswar

Orcid

-

First Name

Debidatta

Last Name

Singhsamanta

MiddleName

-

Affiliation

PhD Scholar, Central Research Laboratory, Institute of Dental Sciences, Siksha O Anusandhan (deemed to be) University, Bhubaneswar, Odisha, India

Email

debidattasinghsamanta@gmail.com

City

Bhubaneswar

Orcid

-

Volume

5

Article Issue

1

Related Issue

45807

Issue Date

2024-02-01

Receive Date

2023-09-26

Publish Date

2024-02-01

Page Start

336

Page End

346

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 5_Issue 1_Pages 336-346.pdf

PDF . 600.0kB

Link

https://mid.journals.ekb.eg/article_320661.html

Detail API

https://mid.journals.ekb.eg/service?article_code=320661

Order

35

Type

Review Article

Type Code

1,160

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Exploring rapid molecular methods for diagnosing <i>Candida</i> species infecting humans: A narrative review.

Details

Type

Article

Created At

25 Dec 2024