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337689

Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of <i>bla</i>NDM-1 and <i>bla</i>KPC genes among Gram negative isolates

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Molecular microbiology

Abstract

Background:  New Delhi Metallo-b-lactamase (NDM- 1) and Klebsiella pneumoniae carbapenemase (KPC) are enzymes associated with resistance to many β- lactam agents. Their early detection is very important for controlling the spread of drug- resistant bacteria. This study aimed to evaluate the use of LOOP-mediated isothermal amplification technique (LAMP) assay for rapid and cost-effective detection of blaNDM-1 and blaKPC genes among Gram-negative bacteria in comparison with conventional PCR. Methods: A total of 156 gram-negative bacterial isolates [Escherichia coli (43), Klebsiella spp. (66), Pseudomonas spp. (47)] were screened for the presence of carbapenemases (blaNDM-1 and blaKPC) using molecular methods such as conventional PCR and LAMP assay. Results:  blaNDM1 was positive in 94/156 (60.2%) isolates and blaKPC was positive in 37/156 (23.7%) isolates by conventional PCR and LAMP. Conclusion: The LAMP technique is an excellent option for the rapid detection of blaNDM-1 and blaKPC genes. The amplification is faster and cheaper than other molecular techniques. It is easy to implement. The thermocycler is not necessary.

DOI

10.21608/mid.2024.255029.1711

Keywords

LAMP, PCR, blaNDM-1, blaKPC

Authors

First Name

Ayat

Last Name

Mohammed

MiddleName

H.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

dr.ayat1200@yahoo.com

City

-

Orcid

-

First Name

Ehsan

Last Name

Hassan

MiddleName

A

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

dr_ehsan66@aun.edu.eg

City

Assiut

Orcid

-

First Name

Mona

Last Name

Hassan

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

monaamin@aun.edu.eg

City

-

Orcid

-

First Name

Ahmed

Last Name

Reda

MiddleName

-

Affiliation

Department of Urology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

ahmedreda_leo@yahoo.com

City

-

Orcid

-

First Name

Shrouk

Last Name

Elghazaly

MiddleName

M.

Affiliation

Intern doctor, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

shm98771@gmail.com

City

-

Orcid

-

First Name

Shereen

Last Name

Mohammed

MiddleName

M.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

mokhdyd@aun.edu.eg

City

-

Orcid

-

Volume

5

Article Issue

1

Related Issue

45807

Issue Date

2024-02-01

Receive Date

2023-12-19

Publish Date

2024-02-01

Page Start

260

Page End

269

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 5_Issue 1_Pages 260-269.pdf

PDF . 537.6kB

Link

https://mid.journals.ekb.eg/article_337689.html

Detail API

https://mid.journals.ekb.eg/service?article_code=337689

Order

28

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Loop-mediated isothermal amplification assay versus polymerase chain reaction for detection of <i>bla</i>NDM-1 and <i>bla</i>KPC genes among Gram negative isolates

Details

Type

Article

Created At

25 Dec 2024