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337691

Cytokines profile and their related genotypes in COVID-19: Correlation with disease severity and outcome in Egyptian patients

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Infectious diseases

Abstract

Background and Aims: COVID-19-related pulmonary inflammation is linked to elevated plasma levels of a group of proinflammatory cytokines. We aim to identify the association between IL-6 rs1800795, IL-17 rs2275913, and IL-37 rs3811046 gene polymorphisms and COVID-19 severity and prognosis. Methods: Two hundreds adult COVID-19-confirmed patients (100 patients with non-severe and 100 patients with severe or critical COVID-19) and 100 healthy individuals were enrolled in this cross-sectional study. Clinical and laboratory evaluations were performed, including liver and kidney functions, complete and differential blood counts, C-reactive protein, and D-dimer. Genotyping for IL-6 (rs1800795), IL-17 (rs2275913), and IL-37 (rs3811046) was conducted using allelic discrimination real-time PCR assay via TaqMan probes. The levels of IL-6, IL-17, and IL-37 were estimated by enzyme-linked immunosorbent assay (ELISA). Results: Serum levels of IL-6 and IL-17 were increased while IL-37 declined with ongoing COVID-19 severity. IL-6 rs1800795 genotypes and alleles did not differ significantly between the studied groups. Meanwhile, IL-17 rs2275913 GA (heterozygous) and AA (homozygous) genotypes and A allele showed significantly higher frequencies in the control group compared to those in the patients' groups and were proposed as protective factors against COVID-19 occurrence and increased severity. Notably, IL-37 rs3811046, GT and TT variants, and T allele were more prevalent in the patients' groups than in the control group and might be related to both disease occurrence and progression. Conclusion: Both GG genotype and G allele of IL-17 (rs2275913) and TT genotype and T allele of IL-37 (rs3811046) and their serum levels are potential risk factors for COVID-19 infection and severity, making them excellent disease management targets.

DOI

10.21608/mid.2024.255229.1712

Keywords

COVID-19, Gene polymorphism, Cytokines, severity

Authors

First Name

Naglaa

Last Name

Elabd

MiddleName

Said

Affiliation

Tropical Medicine Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt

Email

naglaa.alabd.12@med.menofia.edu.eg

City

-

Orcid

0000-0001-8786-0190

First Name

Amany

Last Name

Saleh

MiddleName

A.

Affiliation

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt, Medical surgical nursing Department, College of Nursing, Taibah University, KSA

Email

amany.saleh@med.menofia.edu.eg

City

-

Orcid

0000-0003-0182-3343

First Name

Asmaa

Last Name

Elbrolosy

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt

Email

asmaaelbrolosy@yahoo.com

City

-

Orcid

0000-0001-8196-4511

First Name

Reda

Last Name

Ibrahem

MiddleName

-

Affiliation

Public Health and Community Medicine, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt

Email

reda.ibrahim@med.menofia.edu.eg

City

-

Orcid

-

First Name

Noran

Last Name

Aboelkhair

MiddleName

T.

Affiliation

Clinical Pathology Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt

Email

noran.aboelkhair@med.menofia.edu.eg

City

-

Orcid

-

First Name

Mohamed

Last Name

Enar

MiddleName

-

Affiliation

Al Mahala Elkobra fever Hospital, Al Mahala Elkobra, Egypt

Email

mohamedeiener@gmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Elesdoudy

MiddleName

-

Affiliation

Chest Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt

Email

asd198119@yahoo.com

City

-

Orcid

-

First Name

Marwa

Last Name

Allahouny

MiddleName

M.

Affiliation

ICU Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt

Email

marwamounir49@yahoo.com

City

-

Orcid

-

First Name

Mahmoud

Last Name

Rizk

MiddleName

-

Affiliation

Internal Medicine Department, Faculty of Medicine, Benha University, Banha, Egypt

Email

mahmoudrizk70@yahoo.com

City

-

Orcid

-

First Name

Moamena

Last Name

Elhamoly

MiddleName

S.

Affiliation

Tropical Medicine Department, Faculty of Medicine, Menoufia University, Menoufia, Shebin Elkom, Egypt

Email

moamena1@yahoo.com

City

-

Orcid

0000-0002-2018-042X

Volume

5

Article Issue

1

Related Issue

45807

Issue Date

2024-02-01

Receive Date

2023-12-13

Publish Date

2024-02-01

Page Start

53

Page End

65

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 5_Issue 1_Pages 53-65.pdf

PDF . 700.8kB

Link

https://mid.journals.ekb.eg/article_337691.html

Detail API

https://mid.journals.ekb.eg/service?article_code=337691

Order

7

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Cytokines profile and their related genotypes in COVID-19: Correlation with disease severity and outcome in Egyptian patients

Details

Type

Article

Created At

25 Dec 2024