Aim: This study sought to ascertain the prevalence of multidrug resistant strains among E. coli isolates responsible for urinary tract infections (UTIs) that were resistant to frequently used antimicrobial drugs. Methods: Thirty (30) urine samples were collected from Federal Medical Center (FMC) patients who may have had UTIs in Medical CenterBirnin Kebbi. Regular biochemical tests were used to identify all isolates, and testing for antibiotic susceptibility were performed using disc diffusion method. Results: The majority of the isolates were aztreonam and cefotaxime resistant. Cefotaxime and aztreonam resistance rates overall were 81% and 82%, respectively. Almost all isolates were susceptible to ciprofloxacin and tigecycline, with respective resistance rates of 17% and 10%. One in seventeen isolates of E. coli were multidrug resistance (MDR). Conclusion: According to our findings, only a small percentage of the E. coli isolates included in this investigation were MDR. It is strongly advised that antibiotic prescriptions be monitored in accordance with the recommendations. Both in clinical settings and in the population at large, antibiotic use should be closely monitored. In order to reduce infections, infection prevention and control are essential in all healthcare settings.