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287014

Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Antimicrobial resistance

Abstract

Background: Claims from locals in Nigeria hold that Kunun zaki has some medicinal properties. The study was therefore carried out to investigate the inhibitory effect of lactic acid bacteria (LAB) on multidrug resistant diarrheagenic bacteria in human immunodeficiency virus (HIV) patients. Method: Twenty-five stool samples of seropositive  HIV patients from Plateau State Specialist Hospital confirmed to have chronic diarrhea were collected aseptically and bacteria were isolated and identified using microscopic and biochemical techniques. The antibiotics susceptibility tests of the isolates were also carried out using the disc diffusion method to determine drug resistance of the bacteria. The lactic acid bacteria (LAB) used were isolated and identified using standard bacteriological techniques and analytical profile index (API) kits. Diarrheagenic bacteria which showed multiple resistance to antibiotics were tested against lactic acid bacteria using agar well diffusion method. Results: The results showed that Shigella spp (36.0%), Salmonella spp (16.0%) and Escherichia coli (E. coli) (48.0%) were the diarrheagenic bacteria isolated from the HIV patients. The pathogens were most resistant to ampicillin (60%) and least resistant to tarivid (8%).  Lactobacillus brevis, Lactobacillus lactis, Lactobacillus casei and Lactobacillus plantarum isolated from Kunun zaki demonstrated antibacterial activity against the pathogens with the effect of the two lactic acid bacteria (L. lactis Gb3ii and L. plantarum Ar1) being significantly higher than the individual LAB used respectively. Conclusion: Lactic acid bacteria from Kunun zaki had demonstrated antibacterial effects against multidrug resistant pathogens, hence could be potential probiotics for inclusion in the fermentation of Kunun zaki that HIV patients could consume.

DOI

10.21608/mid.2023.175533.1418

Keywords

Kunun zaki, Multidrug resistant bacteria, lactic acid bacteria, HIV patients

Authors

First Name

Anayochukwu

Last Name

Ngene

MiddleName

Chibuike

Affiliation

Department of Microbiology, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Nigeria.

Email

ngene.anayochukwu@mouau.edu.ng

City

-

Orcid

0000-0003-4730-2834

First Name

Solomon

Last Name

Ebuga

MiddleName

Musa

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

msebuga@gmail.com

City

-

Orcid

-

First Name

John

Last Name

Egbere

MiddleName

Otumala

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

egbereo@yahoo.com

City

Jos

Orcid

-

First Name

Collins

Last Name

Odu

MiddleName

Emeka

Affiliation

Department of Biological Sciences, Faculty of Natural Sciences, University of Maiduguri, Nigeria.

Email

colbounty2002@gmail.com

City

-

Orcid

-

First Name

Margaret

Last Name

Danladi

MiddleName

Musa

Affiliation

Department of Microbiology, Plateau State University, Bokkos, Nigeria.

Email

margaretshambor@gmail.com

City

-

Orcid

-

First Name

Isaac

Last Name

Onyimba

MiddleName

Amechi

Affiliation

Department of Science Laboratory Technology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria.

Email

am_isaac2002@yahoo.com

City

-

Orcid

-

First Name

Michael

Last Name

Dashen

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

macvrendashen@yahoo.co.uk

City

-

Orcid

-

First Name

Edo

Last Name

Ochiheche

MiddleName

Godwin

Affiliation

Department of Science Laboratory Technology, Fidei Polytechnic, Gboko, Nigeria.

Email

ochihechegodwin@yahoo.com

City

-

Orcid

-

First Name

Hemen

Last Name

Nguhemen

MiddleName

Winifred

Affiliation

Department of Science Laboratory Technology, Fidei Polytechnic, Gboko, Nigeria.

Email

winifredhemen28@gmail.com

City

-

Orcid

-

First Name

Amos

Last Name

Ogaji

MiddleName

Obaje

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

ogajiamos@gmail.com

City

-

Orcid

-

Volume

4

Article Issue

3

Related Issue

42578

Issue Date

2023-08-01

Receive Date

2022-11-18

Publish Date

2023-08-01

Page Start

1,008

Page End

1,021

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 4_Issue 3_Pages 1008-1021.pdf

PDF . 995.5kB

Link

https://mid.journals.ekb.eg/article_287014.html

Detail API

https://mid.journals.ekb.eg/service?article_code=287014

Order

35

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Invitro inhibitory effect of Lactic acid bacteria isolated from Kunun zaki against multi-drug resistant diarrhogenic bacteria in HIV patients in Jos, Nigeria

Details

Type

Article

Created At

25 Dec 2024