Background:  Burn wound infections represent a major health problem in hospitals as they increase the morbidity and mortality rates. The emergence of multidrug resistant (MDR) organisms in burn units has made the treatment of these infected wounds more difficult. Methods: We did microbiological isolation, identification, and antibiotic susceptibility testing for bacterial isolates in the burn unit and implemented infection control measures. Results: In our study, we isolated 50 (50%) Stapylococcus.aureus (Staph. aureus) isolates, 28 (28%) MDR Pseudomonas.aeruginosa (P. aeruginosa) isolates. 8 (8%) isolates were non MDR P. aeruginosa, 8 (8%) of the isolates were MDR Klebsiella, 2 (2%) were non-MDR, and 4(4%) were E. coli isolates. The percentage of MDR among P. aeruginosa isolates was 77.7%, and the MDR Klebsiella were 80 % of the Klebsiella isolates. Conclusion: The most common bacterial cause of burn wound infection in the burn unit at Tanta University Hospital was Staph. aureus, followed by P. aeruginosa. Multi drug resistant P. aeruginosa and MDR Klebsiella play a role in burn wound infections in the burn unit at Tanta University Hospital.