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Molecular detection of carbapenem resistant <i>Klebsiella pneumoniae</i> isolated from clinical specimens in Jos, Nigeria

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Medical bacteriology

Abstract

Background: Klebsiella pneumoniae is among the most frequent opportunistic pathogens causing wide range of infections and the emergence and spread of carbapenem resistant Klebsiella pneumoniae are problems currently threatening global health. The present study aimed to determine the prevalence of carbapenem resistant Klebsiella pneumoniae and to detect carbapenem resistance genes in Jos, Nigeria. Methods: A total of 19 clinical specimens were collected from Jos University Teaching Hospital (JUTH) and National Veterinary Research Institute Vom, Jos, Nigeria. Klebsiella pneumoniae was identified by cultural and biochemical methods. Antibiotic susceptibility test was performed using modified Kirby-Bauer disc diffusion technique. Carbapenem resistant Klebsiella pneumoniae isolates were tested for carbapenemase production and blaKPC, blaVIM,and blaNDM genes using Modified Hodges Test and multiplex PCR respectively. Results: Of the 19 clinical specimens examined, 8(42.10%) were positive for Klebsiella pneumonia and most of the isolates were recovered from urine 5(62.50%), followed by sputum 2(25.00%) and isolates from wounds swab recorded the lowest occurrence, 1(12.50%). Antibiotic susceptibility test showed that isolates were highly resistant to gentamicin (62.50%), ceftriaxone (50.00%), amoxicillin/clavulanic acid (62.50%), ciprofloxacin (37.50%), cefepime (62.50%), clarithromycin (50.00%) and meropenem 3(37.50%). Polymerase chain reaction screening for blaKPC, blaVIM,and blaNDM genes which code for carbapenemases among the 3 meropenem resistant Klebsiella pneumoniae isolates were not detected. Conclusion: The expression of high phenotypic antibiotic resistance recorded in this study could be suggestive of other resistance genes which this study was limited for. Therefore, monitoring of carbapenem resistant Klebsiella pneumoniae with the aim of screening other antibiotic resistance genes is strongly recommended

DOI

10.21608/mid.2022.145897.1329

Keywords

carbapenem, resistance, Klebsiella, pneumoniae, Jos

Authors

First Name

Jude

Last Name

Nkup

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

jnkup@yahoo.com

City

Jos

Orcid

0000-0001-8609-8384

First Name

Sarah

Last Name

Joseph

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

sarahjoe21@gmail.com

City

-

Orcid

-

First Name

Yusuf

Last Name

Agabi

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

yusufagabi@gmail.com

City

Jos

Orcid

https://orcid.org/00

First Name

Veronica

Last Name

David

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

verade21@yahoo.co.uk

City

Jos

Orcid

-

First Name

Zakari

Last Name

Hashimu

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

hashimuzakari1000@gmail.com

City

Jos

Orcid

-

First Name

Caroline

Last Name

Madubulum

MiddleName

Chiamaka

Affiliation

Department of Family Medicine, Faculty of Medical Sciences, University of Jos, Jos, Nigeria

Email

carolinemadu@gmail.com

City

Jos

Orcid

-

First Name

Amanyi

Last Name

David

MiddleName

-

Affiliation

Department of Family Medicine, Faculty of Medical Sciences, University of Jos, Jos, Nigeria

Email

amanyidave@yahoo.com

City

Jos

Orcid

-

First Name

Ujah

Last Name

Otobo

MiddleName

-

Affiliation

Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Jos University Teaching Hospital, Jos, Nigeria. College of Public Health, The University of South Florida, Tampa, USA

Email

otoboujah@yahoo.com

City

Jos

Orcid

-

First Name

Gloria

Last Name

Goyil

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

gloriagoyil@gmail.com

City

Jos

Orcid

-

First Name

Nanya

Last Name

Cirfat

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

cirfatnanya11@gmail.com

City

Jos

Orcid

-

First Name

Joseph

Last Name

Anejo-Okopi

MiddleName

Aje

Affiliation

Federal University of Health Sciences, Utukpo, Benue State, Nigeria.

Email

josephokopi@yahoo.com

City

Otukpo

Orcid

-

First Name

Samirah

Last Name

David

MiddleName

-

Affiliation

Department of Microbiology, Faculty of Natural Sciences, University of Jos, Jos, Nigeria

Email

samirahdave222@gmail.com

City

Jos

Orcid

-

Volume

4

Article Issue

2

Related Issue

40831

Issue Date

2023-05-01

Receive Date

2022-06-20

Publish Date

2023-05-01

Page Start

555

Page End

562

Print ISSN

2682-4132

Online ISSN

2682-4140

Article File

MID_Volume 4_Issue 2_Pages 555-562.pdf

PDF . 646.2kB

Link

https://mid.journals.ekb.eg/article_253584.html

Detail API

https://mid.journals.ekb.eg/service?article_code=253584

Order

25

Type

Original Article

Type Code

1,157

Publication Type

Journal

Publication Title

Microbes and Infectious Diseases

Publication Link

https://mid.journals.ekb.eg/

MainTitle

Molecular detection of carbapenem resistant <i>Klebsiella pneumoniae</i> isolated from clinical specimens in Jos, Nigeria

Details

Type

Article

Created At

25 Dec 2024