Background:The antibiotic resistance, plasmid profile and virulence of Escherichia coli isolated from volunteered females of Elizade University, Ilara-Mokin, Ondo State was studied within a period of twenty months along with a questioner. Methods: A total of 300 samples of urine were collected and processed using standard microbiological culturing techniques. Identification, antibiotic susceptibility (disc diffusion), plasmid analysis, detection of virulence gene and curing of virulence were carried out. Results: The result showed a prevalence rate of 10.5% significant bacteriuria in sexually transmitted disease case. Majority (72.4%) were between 16-22 years (30.25±4.58) and 81% of the students had well educated parents. On a general note, Eschericia coli accounted for 42.5% of the bacteria isolated followed by Staphylococcus aureus (32.5%). All the uropathogens isolated were not susceptible to the commonly prescribed antibiotics; cefuroxime (100%) but mostly susceptible to quinolone (84%) and cephalosporin (81%). Of the twenty-five (25) E. coli isolated from these urine samples, fifteen (15) possess the ability to produce beta-lactamase enzyme. Eleven of these fifteen E. coli isolates (11/15%) were positive for type 1 pili fimbriae adhesin virulence factor and one showed the presence of shiga toxin 1. Ten of these E. coli had plasmid resistance gene. The curing of these resistant plasmid using high temperature incubation (at 42⁰C) showed that six were cured of their resistance. Conclusion: These results showed that there has been no significant change in the prevalence of bacteriuria in the recent years. Guideline to prevent indiscriminate use of antibiotics is needed to prevent the resistance observed in this study.